pulid专利名称：How to suppress the formation of adapter dimers
李虹桥发明人：永友 寛一郎
申请号：JP2018247980日语自我介绍>vaguely
申请日：20181228
公开号：JP2020103230A
palmos公开日：
我愿意i do片尾曲
20200709
专利内容由知识产权出版社提供
摘要：PROBLEM TO BE SOLVED: To provide a novel method and kit for suppressing the production of adapter dimer in a method for preparing a nucleic acid library which can be ud for quence analysis in NGS and the like. The prent invention provides a method for suppressing the formation of an adapter dimer by increasing the concentration of a buffer in a reaction solution ud for terminal repair and terminal adenylation reactions. In a specific embodiment, the prent invention provides the following step: (A) coexisting a buffer of 100 mM or more in a reaction solution for performing terminal repair of a nucleic acid fragment and/or terminal adenylation of a nucleic acid fragment, A method for suppressing the formation of an adapter dimer, which compris the steps of: performing a terminal treatment; and (B) reacting the nucleic acid fragment treated in the step (A) with an adapter to prepare a nucleic acid fragment to which the adapter is added. I will provide a. Furthermore, the prent invention also provides a kit and the like that can be ud in the above method. [Selection diagram] None
申请人：東洋紡株式会社英语学习班
sagging地址：大阪府大阪市北区堂島浜二丁目２番８号
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ancestor国籍：JP
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