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BFA/Monensin mixture(250X)
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Contents: BFA/Monensin mixture(250X)
Catalog Number: CS1002
Concentration: 250X (U at 1X: dilute to 4 μL/mL final concentration)浣熊的英文
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blmHandling Conditions: Wear suitable personal protective equipment and gloves.研究生现场确认时间
Formulation: BFA(0.75mg/ml), Monensin(0.35mg/ml) in Ethanol
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Temperature Limitation: Store at -20°C. For long-term storage, product can also be stored at less than or equal to -70°C. Protect from light.≤
Description The BFA/Monensin mixture (250X) is a mixture of BFA and Monensin. It is suitable for u in assays in which prevention of protein transport to the extracellular space is desired and has been specifically formulated for u as a negative control for the PMA/Ionomycin/BFA/Monensin mixture(250X) (cat#:CS1003). Addition of this mixture to cells in culture inhibits the intracellular protein transport pathway resulting in the accumulation of creted proteins in the lumen of the endoplasmic reticulum and in the Golgi apparatus. This accumulation can then be detected by intracellular staining and flow cytometric analysis.
Applications Reported The BFA/Monensin mixture (250X) has been reported for u in flow cytometric analysis.
Applications Tested The BFA/Monensin mixture (250X) has been tested by treatment of mou splenocytes for 5 to 18 hours in combination with the PMA/Ionomicin mixture (cat#: CS1001) followed by intracellular staining and flow cytometric analysis of IFNγ. This mixture is supplied as a 250X concentrate and can be directly added to culture medium at 1X (4 μL/mL). This product is form
ulated with a solvent that will not freeze at recommended storage temperatures. It is recommended that this product be kept tightly aled and cold at all times to avoid excess evaporation.
Related Products
CS0001 PMA 0.1mg/ml in Ethanol 100ul CS0002 Ionomycin 1mg/ml in Ethanol 50ul CS0003 BFA 4mg/ml in Ethanol 60ul CS0004 Monensin 50mg/ml in Ethanol 100ul CS1001 PMA/Ionomycin mixture(250X) 100ul CS1003 PMA/Ionomycin/BFA/Monensin mixture(250X)100ul
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