微生物多样性(扩增子或16SrDNA测序)分析报告解析-α多样性分析

更新时间:2023-07-01 06:56:05 阅读: 评论:0

微⽣物多样性(扩增⼦或16SrDNA测序)分析报告解析-α多样性分析
微⽣物多样性(扩增⼦/16S rDNA测序)
分析报告内容解析
英文名字翻译器——α多样性分析
主要内容
α多样性指数分析内容及意义1
α多样性分析在论⽂中的描述2
01
α多样性指数分析内容及意义
a)群落⽣态学中研究微⽣物多样性,通过单样品的多样性分析(α[Alpha]多样性)可以反映
微⽣物群落的丰度和多样性,包括⼀系列统计学分析指数估计环境群落的物种丰度和多样性。
b)α多样性指分析主要包括:
love hotel
①菌群丰度(Community richness)指数计算:Chao、Ace指数;
破产姐妹1
②菌群多样性(Community diversity)指数计算:Shannon、Simpson指数。玛利亚 莎拉波娃
六一儿童节快乐的英文——获得指数计算汇总表格。孟母三迁翻译
c)α多样性可视化分析图(来源:指数分析汇总表):
稀释性曲线(Rarefraction Curve);Shannon-Wiener曲线;Rank-Abundance曲线;Specaccum曲线
02
α多样性分析在论⽂中的描述
a)稀释性曲线和Shannon-Wiener曲线结合使⽤:
例如1:Shannon and Rarefaction analysis showed that,although new phylotypes can be obtained by additional quencing,most of the gut microbial diversity in each sample was captured with the curre
左面nt quencing depth.After rarefying the quencing depth among all the samples using bootstrap (XX,XXX reads per sample),Shannon diversity index and rarefaction OTU estimates were calculated. There was no significant difference in the richness(as indicated by rarefaction OTU estimates)and diversity between Group A and Group B in this study.
世上没有免费的午餐如有显著差异:while Group B significantly reduced both the richness and diversity of the microbiota(P<0.05OR Show in fig.)
a)稀释性曲线和Shannon-Wiener曲线结合使⽤:
例如2:The richness of oral bacterial communities in three oral habitats of periodontitis patients and the control cohort were estimated by rarefaction and Chao1,and diversity was estimated by Shannon diversity index and Simpson diversity.The shape of the rarefaction curves indicated new phylotypes would be expected with additional quencing.However,the Shannon diversity index curves of all samples reached plateaus with the current quencing,suggesting that most diversity had already been captured.
b)Rank-abundance曲线:
例如1:Rank-abundance curves reflect the fact that a few dominant phylotypes compri the major proportion of the A communities whereas a more even community is en in XXX.
例如2:Bacterial communities were also similar in their structure, exhibiting comparable trends for both rank frequency and abundance across the soils sampled
c)物种累积曲线:
例如:According to the sample number and species OTUs,we calculated the species accumulation curve of all participants.In this study,the curve had reached a plateau,and the species had no more obvious increa as the sample number incread,which indicated that the sample volume in our study was relatively large enough to reflect the species richness.
d)α多样性指数⽐较:
例如:We compared theα-diversity of microbiota between XX and XX samples using the chao1,ACE,obrved species,Shannon and Simpson index, and found that all five indexes showed significant difference(P=XXX,XXX, XXX,XXX,and XXX,respectively).The XXX samples had a significantly higherα-diversity.talented>pscn
温馨提⽰:
⽂章写作时,使⽤α多样性指数种类和⽅式,需根据⽂章表述需求进⾏选择,因此,α多样性指数的差异分析需根据提供的α多样指数汇总表⾃⾏完成。

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