Method and apparatus for automated carbohydrate ma

更新时间:2023-06-25 01:30:14 阅读: 评论:0

专利名称:Method and apparatus for automated石家庄北大青鸟学校
carbohydrate mapping and quencing
日本留学中介发明人:ROGER A O'NEILL,LOUIS B HOFF,SHAHEER H KHAN
申请号:AU2550395
私人教练培训健身教练学校申请日:19950511
公开号:AU2550395Acondchance
combatant公开日:
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专利内容由知识产权出版社提供
austria>hurt的用法
摘要:Methods and apparatus are disclod for improved mapping and quencing of carbohydrate polymers. In one aspect, the improvement includes a means for coupling two capillary electrophoresis (CE) tubes in such a way so as to (i) efficiently transfer a lected sample component from a first CE capillary to a cond CE capillary or, (ii) introduce a supplementary reagent into the paration path between the two capillaries, e.g., an internal standard, binding agent, enzyme, and the like. In an additional aspect, the invention includes improved apparatus and methods for electrophoresis of labeled carbohydrates in which a sample carbohydrate labeled with a first label is parated by CE and its migration behavior is compared with an internal standard labeled with one or more cond labels which are distinguishable from the first label. In yet another aspect, the invention includes improved method and apparatus for quencing carbohydrates in which a sample mixture is parated in a first CE dimension, a component of the sample mixture is lected, the lected component is incubated with an enzyme reagent, the reaction products are parated in a cond CE dimension, the electrophoretic behavior of the reaction products is correlated with the known specificity of the enzyme reagent,
and the process is repeated to determine the complete quence of the carbohydrate sample.
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申请人:PERKIN-ELMER CORPORATION
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