荧光染料光谱列表
染料名称Excitation(nm)Emission(nm)分子量备注信息
Reactiveandconjugatedprobes
Hydroxycoumarin325386
3
31
Succinimidylester
Aminocoumarin350445
3
30
Succinimidylester
Methoxycoumarin360410
3
17
Succinimidylester
CascadeBlue(375);401423
5
96
Hydrazide
PacificBlue403455
4
06
Maleimide
PacificOrange403551
Luciferyellow425528
NBD466539
2
94
NBD-X
R-Phycoerythrin
(PE)
480;565578
2
40k
PE-Cy5conjugates
480;565;6
50
670
akaCychrome,R670,Tri-Color,Quantum
Red
PE-Cy7conjugates
480;565;7
43
767
Red613480;565613PE-TexasRed
PerCP490675Peridininchlorphyllprotein
TruRed490,675695PerCP-Cy5.5conjugate
FluorX494520
5
87
GEHealthcare
Fluorescein495519
3
89
FITC;pHnsitive
BODIPY-FL503512
TRITC547572
4
44
TRITC
X-Rhodamine570576
5
48
XRITC
Lissamine
RhodamineB
570590
TexasRed589615
6
25
Sulfonylchloride
Allophycocyanin
(APC)
650660
1
04k
APC-Cy7
conjugates
650;755767PharRed
AlexaFluor系列荧光染料
AlexaFluor350343442
4
10
AlexaFluor405401421
1
028
AlexaFluor430434540
7
02
AlexaFluor488499519
6
43
QY0.92
AlexaFluor500503525
7
00
AlexaFluor514517542
7
14
AlexaFluor532530555
7
24
QY0.61
AlexaFluor546561572
1
079
QY0.79
AlexaFluor555553568
1
250
QY0.1
AlexaFluor568579603
7
92
QY0.69
AlexaFluor594591618
8
20
QY0.66
AlexaFluor610610629
1
285
AlexaFluor633632648
1
200
AlexaFluor647652668
1
300
QY0.33
AlexaFluor660663691
1
100
AlexaFluor6806807021
150
AlexaFluor700696719
1
400
AlexaFluor750752776
1
300
AlexaFluor790782804
1
750
Cy系列荧光染料
Cy2489506
7
14
QY0.12
Cy3(512);550
570;(615
)
7
67
QY0.15
Cy3B558
572;(620
)
6
58
QY0.67
Cy3.5581
594;(640
)
1
102
QY0.15
Cy5(625);650670
7
92
QY0.28
Cy5.5675694
1
128
QY0.23
Cy7743767
8
18
QY0.28
核酸相关探针染料
Hoechst33342343483
6
16
AT-lective
DAPI345455AT-lective
Hoechst33258345478
6
24
AT-lective
SYTOXBlue431480
~
400
DNA
ChromomycinA3445575CG-lective
Mithramycin445575
YOYO-1491509
1
271
EthidiumBromide493620
3
94
AcridineOrange503530/640DNA/RNA
SYTOXGreen504523~DNA
600
TOTO-1,TO-PRO-1509533
Vitalstain,TOTO:CyanineDimer
TO-PRO:CyanineMonomer
ThiazoleOrange510530
PropidiumIodide
(PI)
536617
6
68.4
LDS751543;590712;607
4
72
DNA(543ex/712em),RNA(590ex/607em)
7-AAD5466477-aminoactinomycinD,CG-lective
SYTOXOrange547570
~
500
DNA
TOTO-3,TO-PRO-3642661
DRAQ5647681,697
4
13
usableexcitationdownto488
细胞功能探针
Indo-1361/330490/405
1
010
/HighCa++,
Fluo-3506526
8
55
>6
DCFH505535
5
29
2'7'Dichorodihydrofluorescein,
oxidizedform
DHR505534
3
46
Dihydrorhodamine123,oxidizedform,
lightcatalyzesoxidation
SNARF548/579587/635pH6/9
荧光蛋白
QYBRPS
Y66H360442
Y66F360508
EBFP380440
0
.18
0
.27
monomer
EBFP2383448
2
0
monomer
Azurite383447
1
5
monomer
GFPuv385508
T-Sapphire399511
0
.60
2
6
2
5
weakdimer
Cerulean433475
0
.62
2
7
3
6
weakdimer
mCFP433475
0
.40
1
3
6
4
monomer
ECFP434477
0
.15
3
CyPet435477
0
.51
1
8
5
9
weakdimer
Y66W436485
mKeima-Red440620
0
.24
3monomer
TagCFP458480
2
9
dimer
AmCyan1458489
0
.75
2
9
tetramer
mTFP1462492
5
4
dimer
S65A471504
MidoriishiCyan472495
0
.9
2
5
dimer
WildTypeGFP396,475508
2
6k
0
.77
S65C479507
TurboGFP482502
2
6k
0
.53
3
7
dimer
TagGFP482505
3
4
monomer
S65L484510
Emerald487509
0
.68
3
9
0
.69
weakdimer;
(Invitrogen)
S65T488511
EGFP488507
2
6k
0
.60
3
4
1
74
weakdimer
AzamiGreen492505
0
.74
4
1
monomer
ZsGreen1493505104tetramer
05k.910
TagYFP508524
4
7
monomer
EYFP514527
2
6k
0
.61
5
1
6
0
weakdimer
Topaz514527
5
7
monomer
Venus515528
0
.57
5
3
1
5
weakdimer
mCitrine516529
0
.76
5
9
4
9
monomer
YPet517530
0
.77
8
0
4
9
weakdimer
TurboYFP525538
2
6k
0
.53
1
.65
dimer
ZsYellow1529539
0
.65
1
3
tetramer
KusabiraOrange548559
0
.60
3
1
monomer
mOrange548562
0
.69
4
9
9monomer
mKO548559
0
.60
3
1
1
22
monomer
TurboRFP553574
2
6k
0
.67
6
2
dimer
tdTomato554581
0
.69
9
5
9
8
tandemdimer
TagRFP555584
5
0
monomer
DsRedmonomer556586
~
28k
0
.1
3
.5
1
6
monomer
DsRed2("RFP")563582
~
110k
0
.55
2
4
mStrawberry574596
0
.29
2
6
1
5
monomer
TurboFP602574602
2
6k
0
.35
2
6
dimer
AsRed2576592~01tetramer
110k.213
mRFP1584607
~
30k
0
.25
monomer
J-Red584610
0
.20
8
.8
1
3
dimer
mCherry587610
0
.22
1
6
9
6
monomer
HcRed1588618
~
52k
0
.03
0
.6
dimer
Katusha58863523dimer
mKate(TagFP635)588635
1
5
monomer
TurboFP635588635
2
6k
0
.34
2
2
dimer
mPlum590649
0
.10
4
.1
5
3
mRaspberry598625
0
.15
1
3
monomer;
faster
photobleach
thanmPlum
其他探针
Monochlorobimane380461
2
26
Glutathioneprobe
Calcein496517
6
23
pH>5
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